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The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage

Received: 26 October 2021     Accepted: 22 November 2021     Published: 29 November 2021
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Abstract

Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.

Published in Advances in Applied Physiology (Volume 6, Issue 2)
DOI 10.11648/j.aap.20210602.15
Page(s) 47-52
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Ovarian Cells, Autophagy, Apoptosis, Sirtuins, Resveratrol, Ex-527, Systemic Autoimmune Damage

References
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    Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. (2021). The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Advances in Applied Physiology, 6(2), 47-52. https://doi.org/10.11648/j.aap.20210602.15

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    Mariia Stupchuk; Alina Lytvynenko; Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv. Appl. Physiol. 2021, 6(2), 47-52. doi: 10.11648/j.aap.20210602.15

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    AMA Style

    Mariia Stupchuk, Alina Lytvynenko, Tetyana Voznesenska. The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage. Adv Appl Physiol. 2021;6(2):47-52. doi: 10.11648/j.aap.20210602.15

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  • @article{10.11648/j.aap.20210602.15,
      author = {Mariia Stupchuk and Alina Lytvynenko and Tetyana Voznesenska},
      title = {The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage},
      journal = {Advances in Applied Physiology},
      volume = {6},
      number = {2},
      pages = {47-52},
      doi = {10.11648/j.aap.20210602.15},
      url = {https://doi.org/10.11648/j.aap.20210602.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20210602.15},
      abstract = {Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - The Effect of Sirtuin 1 Inhibitor Ex-527 and Activator Resveratrol on the Oocytes’ Cells Viability in Mice Model of Experimental Systemic Autoimmune Damage
    AU  - Mariia Stupchuk
    AU  - Alina Lytvynenko
    AU  - Tetyana Voznesenska
    Y1  - 2021/11/29
    PY  - 2021
    N1  - https://doi.org/10.11648/j.aap.20210602.15
    DO  - 10.11648/j.aap.20210602.15
    T2  - Advances in Applied Physiology
    JF  - Advances in Applied Physiology
    JO  - Advances in Applied Physiology
    SP  - 47
    EP  - 52
    PB  - Science Publishing Group
    SN  - 2471-9714
    UR  - https://doi.org/10.11648/j.aap.20210602.15
    AB  - Introduction: Female infertility is a very common pathology for women of reproductive age with autoimmune disorders, which are known to be followed by the abnormal increment of reactive oxygen species levels, moreover, further contributing to follicular atresia and aging of oocytes in the ovaries what directly leads to infertility. Thus, in search for strategies of preventing oxidative threat to ovaries, the current study was aimed to assess the influence of sirtuin 1, a key cellular metabolism regulator and oxidative stress, activator/blocker on the viability of the follicular environment of oocytes (FEO) cells under conditions of experimental systemic autoimmune damage (ESAD). Methods: The study was performed using the model of ESAD on female mice. The FEO cells were cultivated in different conditions: resveratrol 20 µM, Ex-527 20 µM. After 24h, the cells were examined for viability; the ways of cell death via apoptosis, necrosis were estimated using the method of in vivo dual-color with fluorescent nucleic acid dyes Hoechst 33342 and propidium iodide, and the levels of autophagy were estimated using the autophagic vacuoles labeling with monodansylcadaverine assay. Results: The obtained data indicate that a specific inhibitor of sirtuin 1 Ex-527 (20 µM) in vitro inhibits the viability of cells of the FEO cells and increases the percent of cell death via autophagy, apoptosis, and necrosis. On the contrary, the activator of sirtuin 1 - resveratrol, led to an improvement of the viability status of FEO cells, while reducing the negative impact of the inflammatory process. Unidirectional action of Ex-527 and resveratrol compounds at the cellular level has been established. Conclusion: Thus, the results of the present study suggest the involvement of sirtuin 1 in the regulation of the damaging effect of reactive oxygen species on female ovarian cells under the conditions of experimental systemic autoimmune damage.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine

  • Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine

  • Department of Immunophysiology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine

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